Released | 2021-05-26Deposited: 2020-12-13 |
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Title | Spinach variant bound to DFHBI-1T |
Authors | Truong L, Trachman RJ, Ferre-D'Amare AR |
Method | X-RAY DIFFRACTION2.1 Å Rwork: 0.1959 Rfree: 0.2324 C 1 2 1a=150.564 Å b=48.692 Å c=30.612 Å α=90° β=91.364° γ=90° Software: PHASER, PHENIX, XDS |
Primary Citation | Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET. Jeng SCY, Trachman III RJ, Weissenboeck F, Truong L, Link KA, Jepsen MDE, Knutson JR, Andersen ES, Ferre-D'Amare AR, Unrau PJ RNA (2021) |
Entry Content | Deposited MW: 23.64 kDa |
Analysis | |
NA Annotations | |
Ligand Annotations |
Type | Description (hover for sequence) | Chains(Auth) | Source | MW(kDa) | Links |
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RNA | RNA (69-MER) | G | synthetic construct | 22.4 | |
Ligand | (5Z) 5 (3,5 difluoro 4 hydroxybenzylidene) 2 methyl 3 (2,2,2 trifluoroethyl) 3,5 dihydro 4H imidazol 4 one | 0.32 | |||
SPERMINE | 0.202 | ||||
PHOSPHATE ION | 0.095 | ||||
POTASSIUM ION | 0.039 | ||||
MAGNESIUM ION | 0.024 | ||||
SODIUM ION | 0.023 |
Assembly # | Type, Components (Author Chain Ids) | Oligomer | Point Symmetry | Actions |
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1 | author defined RNA: RNA (69-MER) (G) | monomeric(1) | . | View Assembly in 3D (Mol*) View Nucleic Acid Parameters (DSSR) Download Assembly mmCIF File (RCSB) |