Released | 2016-05-18Deposited: 2016-03-24 |
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Title | Human holo-PIC in the initial transcribing state (no IIS) |
Authors | He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E |
Method | ELECTRON MICROSCOPY6.3 Å SINGLE PARTICLESoftware: RELION |
Primary Citation | Near-atomic resolution visualization of human transcription promoter opening. He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E Nature (2016) |
Entry Content | Deposited MW: 1182.77 kDa |
Analysis | |
Protein Annotations |
Type | Description (hover for sequence) | Chains(Auth) | Source | MW(kDa) | Links |
---|---|---|---|---|---|
DNA | SCP-X (83-MER) | X | Homo sapiens | 25.8 | |
SCP-Y (83-MER) | Y | Homo sapiens | 25.4 | ||
RNA | RNA (6-MER) | Z | Homo sapiens | 1.9 | |
Protein | DNA-directed RNA polymerase II subunit RPB1 | A | Homo sapiens | 217.4 | |
DNA-directed RNA polymerase II subunit RPB2 | B | Homo sapiens | 134.1 | ||
DNA-directed RNA polymerase II subunit RPB3 | C | Homo sapiens | 31.5 | ||
DNA-directed RNA polymerase II subunit RPB4 | D | Homo sapiens | 16.3 | ||
DNA-directed RNA polymerase II subunit RPB5 | E | Homo sapiens | 24.6 | ||
DNA-directed RNA polymerase II subunit RPB6 | F | Homo sapiens | 14.5 | ||
DNA-directed RNA polymerase II subunit RPB7 | G | Homo sapiens | 19.3 | ||
DNA-directed RNA polymerase II subunit RPB8 | H | Homo sapiens | 17.2 | ||
DNA-directed RNA polymerase II subunit RPB9 | I | Homo sapiens | 14.5 | ||
DNA-directed RNA polymerase II subunit RPB10 | J | Homo sapiens | 7.7 | ||
DNA-directed RNA polymerase II subunit RPB11-a | K | Homo sapiens | 13.3 | ||
DNA-directed RNA polymerase II subunit RPB12 | L | Homo sapiens | 7 | ||
Transcription initiation factor IIB | M | Homo sapiens | 34.9 | ||
Transcription initiation factor IIA subunit 1 | N | Homo sapiens | 41.5 | ||
Transcription initiation factor IIA subunit 2 | O | Homo sapiens | 12.5 | ||
TATA-box-binding protein | P | Homo sapiens | 37.7 | ||
General transcription factor IIE subunit 1 | Q | Homo sapiens | 49.5 | ||
Transcription initiation factor IIE subunit beta | R | Homo sapiens | 33.1 | ||
General transcription factor IIF subunit 1 | S | Homo sapiens | 58.3 | ||
General transcription factor IIF subunit 2 | T | Homo sapiens | 28.4 | ||
TFIIH basal transcription factor complex helicase XPB subunit | V | Homo sapiens | 89.4 | ||
TFIIH basal transcription factor complex helicase XPD subunit | W | Homo sapiens | 87 | ||
General transcription factor IIH subunit 2 | 0 | Homo sapiens | 44.5 | ||
General transcription factor IIH subunit 5 | 1 | Homo sapiens | 8.1 | ||
General transcription factor IIH subunit 4 | 2 | Homo sapiens | 52.2 | ||
General transcription factor IIH subunit 3 | 3 | Homo sapiens | 34.4 | ||
Ligand | ZINC ION | 0.065 | |||
MAGNESIUM ION | 0.024 |
Assembly # | Type, Components (Author Chain Ids) | Oligomer | Point Symmetry | Actions |
---|---|---|---|
1 | author defined DNA: SCP-X (83-MER) (X) DNA: SCP-Y (83-MER) (Y) Protein: DNA-directed RNA polymerase II subunit RPB1 (A) Protein: DNA-directed RNA polymerase II subunit RPB10 (J) Protein: DNA-directed RNA polymerase II subunit RPB11-a (K) Protein: DNA-directed RNA polymerase II subunit RPB12 (L) Protein: DNA-directed RNA polymerase II subunit RPB2 (B) Protein: DNA-directed RNA polymerase II subunit RPB3 (C) Protein: DNA-directed RNA polymerase II subunit RPB4 (D) Protein: DNA-directed RNA polymerase II subunit RPB5 (E) Protein: DNA-directed RNA polymerase II subunit RPB6 (F) Protein: DNA-directed RNA polymerase II subunit RPB7 (G) Protein: DNA-directed RNA polymerase II subunit RPB8 (H) Protein: DNA-directed RNA polymerase II subunit RPB9 (I) Protein: General transcription factor IIE subunit 1 (Q) Protein: General transcription factor IIF subunit 1 (S) Protein: General transcription factor IIF subunit 2 (T) Protein: General transcription factor IIH subunit 2 (0) Protein: General transcription factor IIH subunit 3 (3) Protein: General transcription factor IIH subunit 4 (2) Protein: General transcription factor IIH subunit 5 (1) Protein: TATA-box-binding protein (P) Protein: TFIIH basal transcription factor complex helicase XPB subunit (V) Protein: TFIIH basal transcription factor complex helicase XPD subunit (W) Protein: Transcription initiation factor IIA subunit 1 (N) Protein: Transcription initiation factor IIA subunit 2 (O) Protein: Transcription initiation factor IIB (M) Protein: Transcription initiation factor IIE subunit beta (R) RNA: RNA (6-MER) (Z) | 29-meric(29) | C1 | View Assembly in 3D (Mol*) View Nucleic Acid Parameters (DSSR) Download Assembly mmCIF File (RCSB) |