Released | 2017-02-15Deposited: 2016-08-18 |
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Title | Crystal structure of nucleosome particle in the presence of human testis-specific histone variant, hTh2b |
Authors | Kumarevel T, Sivaraman P |
Method | X-RAY DIFFRACTION2.91 Å Rwork: 0.2 Rfree: 0.258 P 21 21 21a=106.887 Å b=110.078 Å c=182.811 Å α=90° β=90° γ=90° Software: CNS, HKL-2000, PDB_EXTRACT, PHENIX |
Primary Citation | Structural analyses of the nucleosome complexes with human testis-specific histone variants, hTh2a and hTh2b Padavattan S, Thiruselvam V, Shinagawa T, Hasegawa K, Kumasaka T, Ishii S, Kumarevel T Biophys Chem (2017) |
Entry Content | Deposited MW: 200.23 kDa |
Analysis | |
NA Annotations | |
Protein Annotations |
Type | Description (hover for sequence) | Chains(Auth) | Source | MW(kDa) | Links |
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DNA | DNA (146-MER) | I,J | Homo sapiens | 45.1 | |
Protein | Histone H3.1 | A,E | Homo sapiens | 15.3 | |
Histone H4 | B,F | Homo sapiens | 11.3 | ||
Histone H2A type 1-D | C,G | Homo sapiens | 14 | ||
Histone H2B type 1-A | D,H | Homo sapiens | 14.1 | ||
Ligand | MANGANESE (II) ION | 0.055 | |||
CHLORIDE ION | 0.035 |
Assembly # | Type, Components (Author Chain Ids) | Oligomer | Point Symmetry | Actions |
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1 | author and software defined DNA: DNA (146-MER) (I,J) Protein: Histone H2A type 1-D (C,G) Protein: Histone H2B type 1-A (D,H) Protein: Histone H3.1 (A,E) Protein: Histone H4 (B,F) | decameric(10) | C2 | View Assembly in 3D (Mol*) View Nucleic Acid Parameters (DSSR) Download Assembly mmCIF File (RCSB) |