Released | 2015-09-23Deposited: 2014-11-27 |
---|---|
Title | Crystal structure of the nucleosome core particle |
Authors | Sivaraman P, Kumarevel TS |
Method | X-RAY DIFFRACTION2.805 Å Rwork: 0.2214 Rfree: 0.27 P 21 21 21a=105.381 Å b=109.363 Å c=175.572 Å α=90° β=90° γ=90° Software: HKL-2000, PDB_EXTRACT, PHASER, PHENIX |
Primary Citation | Structural and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming Padavattan S, Shinagawa T, Hasegawa K, Kumasaka T, Ishii S, Kumarevel T Biochem Biophys Res Commun (2015) |
Entry Content | Deposited MW: 199.42 kDa |
Analysis | |
NA Annotations | |
Protein Annotations |
Type | Description (hover for sequence) | Chains(Auth) | Source | MW(kDa) | Links |
---|---|---|---|---|---|
DNA | DNA (146-MER) | I,J | synthetic construct | 45.1 | |
Protein | Histone H3.1 | A,E | Homo sapiens | 15.3 | |
Histone H4 | B,F | Homo sapiens | 11.3 | ||
Histone H2A type 1-B/E | C,G | Homo sapiens | 14 | ||
Histone H2B type 1-B | D,H | Homo sapiens | 13.9 | ||
Ligand | MANGANESE (II) ION | 0.055 | |||
CHLORIDE ION | 0.035 |
Assembly # | Type, Components (Author Chain Ids) | Oligomer | Point Symmetry | Actions |
---|---|---|---|
1 | author and software defined DNA: DNA (146-MER) (I,J) Protein: Histone H2A type 1-B/E (C,G) Protein: Histone H2B type 1-B (D,H) Protein: Histone H3.1 (A,E) Protein: Histone H4 (B,F) | decameric(10) | C2 | View Assembly in 3D (Mol*) View Nucleic Acid Parameters (DSSR) Download Assembly mmCIF File (RCSB) |