Released | 2006-07-04Deposited: 2006-05-12 |
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Title | Ternary complex of hPoli with DNA and dCTP |
Authors | Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK |
Method | X-RAY DIFFRACTION2.3 Å Rwork: 0.22815 Rfree: 0.27818 P 65 2 2a=98.148 Å b=98.148 Å c=203.112 Å α=90° β=90° γ=120° Software: CNS, DENZO, REFMAC, SCALEPACK |
Primary Citation | Hoogsteen base pair formation promotes synthesis opposite the 1,N(6)-ethenodeoxyadenosine lesion by human DNA polymerase iota. Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK Nat Struct Mol Biol (2006) |
Entry Content | Deposited MW: 52.41 kDa |
Analysis | |
Protein Annotations | |
Nonstandard Nucleotides |
Type | Description (hover for sequence) | Chains(Auth) | Source | MW(kDa) | Links |
---|---|---|---|---|---|
DNA | AGGACCC (7-MER) | P | 2.1 | ||
TAGGGTCCT (9-MER) | T | 2.8 | |||
Protein | DNA polymerase iota | A | Homo sapiens | 47 | |
Ligand | 2' DEOXYCYTIDINE 5' TRIPHOSPHATE | 0.467 | |||
MAGNESIUM ION | 0.024 |
Assembly # | Type, Components (Author Chain Ids) | Oligomer | Point Symmetry | Actions |
---|---|---|---|
1 | author defined DNA: AGGACCC (7-MER) (P) DNA: TAGGGTCCT (9-MER) (T) Protein: DNA polymerase iota (A) #Symmetry Operations to Build: 2 | hexameric(6) | C2 | View Assembly in 3D (Mol*) View Nucleic Acid Parameters (DSSR) Download Assembly mmCIF File (RCSB) |